SCDLP Broth for Pre-culture of Cosmetic Samples
SCDLP Broth is a liquid enrichment medium with neutralizers, specially applied for bacterial pre-culture before cosmetic sample testing. It can neutralize residual preservatives in cosmetics so that target bacteria can recover and multiply normally.
Formula Composition (per Liter)
| Ingredient |
Content |
| Casein peptone |
17.0 g |
| Soybean peptone |
3.0 g |
| Sodium chloride |
5.0 g |
| Dipotassium hydrogen phosphate |
2.5 g |
| Glucose |
2.5 g |
| Lecithin |
1.0 g |
| Final pH |
7.2 ± 0.1 at 25 ℃ |
Inspection Principle
Casein peptone and soybean peptone supply nitrogen sources, vitamins and growth factors for bacterial growth. Sodium chloride maintains osmotic balance. Dipotassium hydrogen phosphate acts as buffering agent. Glucose serves as fermentable carbohydrate. Lecithin and Tween 80 work as neutralizers and emulsion dispersants. Lecithin neutralizes quaternary ammonium salts; Tween 80 neutralizes phenols, hexachlorophenols and formalin. The combination of the two neutralizes ethanol.
Directions for Use
Weigh 31.0 g of this medium powder and add an extra 7.0 g Tween‑80. Heat and dissolve in 1000 mL distilled water, dispense into containers, autoclave at 121 ℃ for 15 minutes, and reserve for later use.
Microbial Performance Test for SCDLP Broth
Prepare the medium following the instructions. Inoculate the specified quality control strains and incubate aerobically at 36 ± 1 ℃ for 18–24 hours.
