Cell-Free DNA&RNA Collection Tube （in room temperature）

Product Introduction
1.The sputum/alveolar lavage fluid free DNA&RNA (cell free DNA and cell free RNA) storage tube at room temperature is a collection device that can be directly used for the collection, transportation, and preservation of cell free DNA and cell free RNA in sputum/alveolar lavage fluid. This storage tube can stably preserve genomic DNA and RNA in sputum/alveolar lavage fluid cells simultaneously;
2.Cell free DNA and cell free RNA in the sample may be damaged due to processing, transportation, and extraction of the sample, leading to nucleated cell lysis and subsequent release of cell DNA and RNA. At the same time, cell free DNA and cell free RNA degradation may also occur due to nuclease activity;
3.The preservation solution components can effectively inhibit the degradation of cell free DNA and cell free RNA by nucleases, and maintain the integrity of nucleated cells, avoiding the release of genomic DNA and RNA in cells that may affect the accuracy of detection results;
4.The sputum/alveolar lavage fluid samples in this storage tube can be directly extracted from cell free DNA and cell free RNA or genomic DNA and RNA from nucleated cells using conventional commercial reagent kits, and then applied to downstream molecular diagnosis detection and analysis, such as PCR, Southern Blot, NGS, etc.

Ordering information: 10mL, 50 pics per box

Product advantages:

1.Convenient sample collection, transportation, and storage; DNA, RNA, cell free DNA, and cell free RNA in samples can be stably stored for up to 7 days at room temperature of 4-37 ℃; Suitable for gene detection fields such as PCR and high-throughput sequencing.
2、 High quality and stability
Preserve cell free DNA and cell free RNA in whole blood at room temperature for 7 days, suitable for gene detection fields such as tumor liquid biopsy and unknown infections
Reduce repeated blood draws for patients, dual use with one tube
Stable negative pressure and dedicated blood collection vessels suitable for use in high altitude areas
High quality glass material, greatly reducing the risk of pipe breakage and cracking
Valid for up to 18 months

3、 Professional and meticulous after-sales service
Under extreme external transportation temperature conditions (below 4 ℃ and above 37 ℃), supporting solutions can be provided
R&D products can be customized with personalized outer packaging, providing a highly competitive price advantage.

Product performance comparison
The number of sequencing reads for microorganisms is shown in the following figure


Product validation data
 1.Agilent 2100 detection of cf-DNA and cf-RNA
Agilent 2100 Bioanalyzer detects the concentration and integrity of free nucleic acids (cell free DNA and cell free RNA). Collect peripheral blood samples using Beyondbio , Street, and EDTA tubes and store them at room temperature for 7 days. Separate 1mL of plasma to extract cell free DNA and cell free RNA, respectively. Agilent 2100 test results are visible

Figure 1. When stored at room temperature until the 7th day, the cell free DNA sample concentration and integrity of the Beyondbio  preservation tube were stable.

Figure 2. When the plasma cell free RNA samples in EDTA tubes were stored for 3 days, multiple fragments of varying sizes appeared and the concentration also significantly increased. The concentration in Street tubes was lower than that in Beyondbio  tubes; At 7 days, the concentration and integrity of the Beyondbio  preservation tube cell free RNA sample were still well preserved.

2、 Digital PCR detection of cf-DNA and cf-RNA
Droplet digital PCR was used to detect the copy number changes of c-fos gene in Beyondbio  preservation tubes in their plasma cf-DNA and cf-RNA samples.

Figure 3. C-fos gene copy number results of cf-DNA in Lakebio preservation tube plasma samples. It can be seen that after being stored at room temperature for 7 days, there was no significant fluctuation in the c-fos gene content of cf-DNA samples detected by digital PCR.

Figure 4. The c-fos gene copy number results of plasma samples cf-RNA stored in Beyondbio storage tubes on days 0, 3, and 7 were compared with those in Street and EDTA tubes. The results showed that the c-fos gene quantity of cf-RNA samples stored in Lakebio storage tubes at room temperature for 7 days was very stable.