Taq Antibody
Taq Antibody is an anti Taq antibody used in Hot Start PCR, which inhibits DNA polymerase activity after binding to Taq enzyme. When using this product for PCR amplification, the combination of anti Taq antibodies and Taq enzymes before high-temperature denaturation inhibits DNA polymerase activity, which can effectively inhibit non-specific annealing of primers and non-specific amplification caused by primer dimers under low temperature conditions. The anti Taq antibody undergoes denaturation in the initial DNA denaturation step of the PCR reaction, restoring DNA polymerase activity and achieving the Hot Start PCR effect. This product does not require special anti Taq antibody inactivation treatment and can be used under conventional PCR reaction conditions. The working principle is as follows:
Working principle of taq enzyme antibody
Storage conditions: Low temperature transportation, -20 ℃ storage, valid for one year.
Definition of activity: After mixing Taq Antibody with Taq DNA Polymerase and bathing at 25 ℃ for 10 minutes, the amount of Taq Antibody that inhibits over 90% of the activity of Taq DNA Polymerase at 55 ℃ for 10 minutes is defined as 1U.
Purity:
1. 10 U Taq Antibody and 1 μ G's λ DNA-Hind III reacted at 37 ℃ or 70 ℃ for 1 hour without any changes in the electrophoretic bands of DNA.
2. 10 U Taq Antibody and 1 μ The supercoiled pBR322 DNA of g reacted at 37 ℃ or 70 ℃ for 1 hour, and the electrophoresis bands of the DNA did not change.
3. 10 U Taq Antibody and 1 μ G's λ When DNA is reacted at 37 ℃ or 70 ℃ for 1 hour, the electrophoretic bands of DNA do not change.
4. 10 U Taq Antibody and 1 μ The 16S and 23S rRNAs of g reacted at 37 ℃ or 70 ℃ for 1 hour, and the electrophoretic bands of the RNA did not change.
Under the PCR reaction conditions of 35 Cycles, there is no contamination of Mouse Genomic DNA.
