Taq Antibody
Taq Antibody is an anti‑Taq antibody specially developed for Hot Start PCR. It binds to Taq DNA polymerase and inhibits polymerase activity.
Before high-temperature denaturation in PCR amplification, the antibody-Taq complex suppresses enzyme activity, effectively preventing low-temperature non-specific primer annealing and non-specific amplification induced by primer dimers. The antibody is denatured in the first DNA denaturation stage of PCR, releasing active Taq DNA polymerase to realize Hot Start PCR. No separate inactivation procedure is required; it is compatible with standard PCR reaction systems.
Working Principle
Anti‑Taq antibody binds Taq DNA polymerase and inhibits enzymatic activity at low temperature. The antibody loses binding function after high-temperature denaturation, and Taq polymerase regains activity to start normal amplification.
Storage
Transport under low temperature; store at -20 ℃. Shelf life: 1 year.
Activity Definition
1 Unit (1U) is defined as the amount of Taq Antibody that inhibits more than 90% of Taq DNA Polymerase activity after mixing with Taq polymerase and incubating at 25 ℃ for 10 min, followed by reaction at 55 ℃ for 10 min.
Purity Specifications
- 10 U Taq Antibody + 1 μg λ DNA‑Hind III, incubated at 37 ℃ or 70 ℃ for 1 h: DNA electrophoresis bands remain unchanged.
- 10 U Taq Antibody + 1 μg supercoiled pBR322 DNA, incubated at 37 ℃ or 70 ℃ for 1 h: DNA electrophoresis bands remain unchanged.
- 10 U Taq Antibody + 1 μg λ DNA, incubated at 37 ℃ or 70 ℃ for 1 h: DNA electrophoresis bands remain unchanged.
- 10 U Taq Antibody + 1 μg 16S & 23S rRNA, incubated at 37 ℃ or 70 ℃ for 1 h: RNA electrophoresis bands remain unchanged.
Contamination Test
No mouse genomic DNA contamination detected after 35 PCR cycles.
