High tolerance aganist various reaction inhibitors
► Reduce nonspecific primer annealing
► Plays in efficient PCR amplification of GC-rich sequences
► Using the same protocol and cycling conditions as conventional Taq DNA polymerases
Taq DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and also possesses a 5´→3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures, but is not recommended for use in DNA sequencing reactions. Our Taq DNA Polymerase is provided in the storage buffer compatible with any reaction buffer designed for Taq DNA polymerase, which containing 20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% Nonidet-P40 and 0.5% Tween 20.
10X Reaction Buffer without MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton X-100. Coupled with separate 25mM MgCl2 Solution.
10X Reaction Buffer with MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C), 1% Triton X-100 and 15mM MgCl2. Optimized for use with 0.2mM of each dNTP.
Features
Reliable: Compositions of the storage buffer have been optimized to assure quality performance of the enzyme under a variety of conditions.
Flexible: Choose either Taq with 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2.
Performance Guarantee: Our enzymes and reagents are proven in PCR to ensure reliable, high-performance results.