Tissue Digestion Solution can gently and quickly digest normal tissue samples into cell suspensions or cell clumps, which can be used for subsequent organoid construction. This tissue digestive fluid is widely used in various normal tissue specimens of mammals, (such as the intestine, liver, lungs, stomach, etc)Digestion and dissociation of pancreas before primary culture in vitro.
Product specifications:100mL;500mL
Storage and use of primary tissue digestive fluid Long term storage at -20 ° C, valid for one year. After thawing, it can be stably stored at 4 ° C for 14 days. It can be thawed at room temperature or overnight at 4 ° C to avoid repeated freeze-thaw cycles. It is recommended to store it at -20 ° C after first receiving the product and packaging it under sterile conditions. Attention: Fungal and bacterial antibiotics have been added to this product. Use of Primary Tissue Digestive Fluid Attention: Research involving primary human tissue samples must comply with all government regulations and relevant regulations. Prior to collecting primary human tissue samples, informed consent must be obtained from all participants. 1. Transfer the primary tissue sample directly to a pre cooled tissue transport preservation solution (2-8 ° C), and the sample needs to be completely covered by the primary tissue preservation solution (if the sample has already been placed in another buffer or culture medium, please replace the entire solution with the pre cooled tissue transport preservation solution). 2. During transportation, the sample storage tube should be placed in a low temperature environment of 2-8 ° C (such as an insulation box containing ice bags). 3. At the end of storage or transportation, samples are taken from the tissue transport preservation solution and used for subsequent experiments, such as using organoid culture kits for tumor or normal tissue organoid establishment.
Organoids refer to tissue analogues with a certain spatial structure formed by three-dimensional cultivation of adult stem cells or pluripotent stem cells in vitro. Organs are highly consistent with their source tissues in terms of tissue structure, cell type, self-renewal ability, and function, thus demonstrating unique advantages in multiple biomedical fields such as developmental biology, disease modeling, precision medicine, drug research and development, gene and cell therapy, infection and immunity, and regenerative medicine. Product Description: Tissue Digestion Solution can gently and quickly digest normal tissue samples into cell suspensions or cell clumps, which can be used for subsequent organoid construction. This tissue digestive fluid is widely used in various normal tissue specimens of mammals, (such as the intestine, liver, lungs, stomach, etc)Digestion and dissociation of pancreas before primary culture in vitro.