• Two-Enzyme One-Tube RT-PCR Kit
  • Two-Enzyme One-Tube RT-PCR Kit

Two-Enzyme One-Tube RT-PCR Kit

This product is a carefully optimized dual enzyme one tube RT-PCR kit based on MMLV reverse transcriptase and Taq DNA polymerase. It contains all RT-PCR reagents except template RNA and its specific primers, including MMLV reverse transcriptase, Taq DNA polymerase, RT-PCR buffer, etc., which can complete RNA → cDNA → PCR reaction (RT-PCR reaction) in the same reaction tube.
$8.50
  • Two-Enzyme One-Tube RT-PCR Kit

SPECIFICATION

                           Two-Enzyme One-Tube RT-PCR Kit
This product is a carefully optimized dual enzyme one tube RT-PCR kit based on MMLV reverse transcriptase and Taq DNA polymerase. It contains all RT-PCR reagents except template RNA and its specific primers, including MMLV reverse transcriptase, Taq DNA polymerase, RT-PCR buffer, etc., which can complete RNA → cDNA → PCR reaction (RT-PCR reaction) in the same reaction tube.
Product features:
1. Complete RT-PCR reaction in one tube to avoid sample cross contamination.
2. Ready to use, users do not need to prepare various reagents required for RT-PCR separately.
3. The main components are only two RT-PCR buffers and MMLV-Taq Mix, which minimizes the sampling steps and greatly reduces the sampling error, increasing repeatability.
4. Widely used, suitable for various RNA templates ranging from viral RNA to human RNA.
5. High sensitivity, each RT-PCR system can detect a minimum of 200 copies of RNA molecules, and can amplify up to 2000 nt of template RNA.
The obtained RT-PCR products can be directly used for AT cloning.
Kit composition:
Component specifications
Double enzyme one tube RT-PCR Buffer (2 ×) seven hundred and fifty μ L
MMLV-Taq Mix 100 μ L
RNase free water 1ml
1 instruction manual
Storage conditions: Low temperature transportation, -20 ℃ storage, valid for one year.
Usage:
Attention: It is best to briefly centrifuge all centrifuge tubes for a few seconds before use to allow the solution on the tube wall to settle to the bottom of the tube. Below is a 30 μ Taking RT-PCR of the L system as an example, if the volume is different, the dosage of each component needs to be adjusted appropriately.
1. In a clean RNase free PCR tube, first add the following components:
Component negative control sample
RNA template (self prepared, divided into the following three situations) not available below
Total RNA without 100-500ng
Or poly (A) mRNA without 10-500ng
No specific RNA obtained from in vitro transcription, 0.01 pg-500ng
RNA specific primer I (self prepared), final concentration 300nM, final concentration 300nM
RNA specific primer II (self prepared) final concentration 300nM final concentration 300nM
Probe (only for Realtime RT-PCR) final concentration 200nM final concentration 200nM
RNase free water replenishment to 13 μ L to 13 μ L
Double enzyme one tube RT-PCR Buffer (2 ×) fifteen μ L 15 μ L
MMLV-Taq Mix 2 μ L 2 μ L
Note: Usually, when using this reagent for the first time, the primer concentration of 300nM and the probe concentration of 200nM can be used for experiments. Based on the specific situation of the experimental results, adjust the primer concentration within the range of 200-800M and adjust the probe concentration within the range of 50-400nM
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