• Universal Plus DNA Library Prep Kit for MGI
  • Universal Plus DNA Library Prep Kit for MGI

Universal DNA Library Prep Kit for Illumina

The Universal Plus DNA Library Prep Kit for Illumina is an ultra fast enzyme digestion DNA library construction kit specifically developed for the MGI high-throughput sequencing platform. This kit simplifies the library construction process, shortens the operation time, takes about 2 hours to construct a single library, and has the characteristics of high library conversion rate and low background bacterial interference. The compatible initial input amount is 100 pg -500 ng,
$1200.00
  • Universal Plus DNA Library Prep Kit for MGI

SPECIFICATION

Product Description:
Universal DNA Library Prep Kit for Illumina is a library construction kit specifically optimized for Illumina's high-throughput sequencing platform, which can convert 100 pg-1 μ The segmented double stranded Input DNA is converted into an Illumina high-throughput sequencing platform specific library. As a new generation upgraded version, this kit significantly improves the conversion rate of low-quality template libraries and reduces library duplication rates through optimization and improvement of the end repair module, connection module, and library amplification module. This kit is widely applicable for PCR or PCR Free library construction of various samples, and is compatible with targeted capture processes. All reagents provided in the kit have undergone strict quality control and functional verification, greatly ensuring the stability and repeatability of library construction.

Product application:
Whole genome sequencing; Full exon or other targeted capture sequencing; Amplifiers sequencing; Immunoprecipitation sequencing; Macrogenomic sequencing; Methylation sequencing

Product advantages:
1. Wide sample compatibility: In addition to conventional samples, it is compatible with low-quality FFPE and cfDNA samples
2. High library building efficiency: higher library conversion rate
3. Accurate mutation detection: accurate low-frequency mutation detection rate

Test Cases
1. Suitable for low-quality FFPE, cfDNA, etc. The ND610 library was constructed using clinical FFPE and cfDNA templates with different inputs. Under the same amplification cycle number, the ND610 library had a significant advantage over similar products from Company A.

2. Higher library conversion rate: Using different samples and inputs for library construction, under different library conversion rate evaluation methods, the library conversion rate of ND610 is significantly better than that of similar products from Company A.

3. Higher effective sequencing depth: On the 1% FFPE tumor SNV standard, the low-frequency mutation sites detected in the library constructed by ND610 are consistent with similar products of Company A; On the 0.1% ctDNA standard for lung cancer, the library constructed by ND610 showed better detection of low-frequency mutation sites than similar products from Company A.


Composition&Description

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