SPECIFICATION
Product Description:
The Universal End Preparation Module for Illumina is a targeted optimization end repair module built for the Illumina high-throughput sequencing platform library. Using this kit can efficiently repair 100 pg-1 μ At the end of fragmented DNA, the repair product contains a phosphate group at the 5 'end and a dA protrusion at the 3' end. Compared with conventional library building methods, the Universal End Preparation Module for Illumina adopts a one-step reaction process that combines end repair and dA tail steps, while reducing the reaction time of each step, significantly reducing the demand for starting DNA templates and shortening experimental time. The reagent adopts the form of Mix, making the pipetting operation more convenient and compatible with automated library building equipment. All reagents provided in the kit have undergone strict quality control and functional verification, greatly ensuring the stability and repeatability of library construction.
Product application:
Whole genome sequencing; Full exon or other targeted capture sequencing; Amplifiers sequencing; Immunoprecipitation sequencing; Macrogenomic sequencing; Methylation sequencing
Product advantages:
1. Single tube end repair with dA tail reaction
2. End repair with A takes less time, only 30 minutes
3. The reagent adopts Mix form and is compatible with automated library building equipment
4. The template has a wider compatibility range and can efficiently repair 100 pg-1 μ G Input DNA
5. The sample type has a wider compatibility range and is suitable for cfDNA, FFPE, and fragmented DNA