The Universal Plus DNA Library Prep Kit for MGI is an ultra fast enzyme digestion DNA library construction kit specifically developed for the MGI high-throughput sequencing platform. This kit simplifies the library construction process, shortens the operation time, takes about 2 hours to construct a single library, and has the characteristics of high library conversion rate and low background bacterial interference. The compatible initial input amount is 100 pg -500 ng, suitable for DNA samples of different types such as alveolar lavage fluid, blood cells, serum, plasma, cerebrospinal fluid, etc., and can be better applied in the field of pathogen detection.
Product advantages1. Fast database construction time - single library construction time is as short as 100 minutes
2. Clean background bacteria - strict control of background nucleic acid
3. Accurate pathogen detection - accurate detection of pathogens in the sample
Reagent performance testing1. Library outputThe UNDM637 is compatible with building libraries with input levels of 0.1-500 ng, and the library output can be achieved under different input levels μ G level, higher than the output of similar product libraries from Supplier A and Supplier B companies.
2. CompatibilityDue to the fact that the components of the two test kits, except for the primers, are completely the same, the compatibility data can refer to the compatibility data of UND637, as follows:
a: Sample compatibilityClinical samples (such as plasma, cerebrospinal fluid, alveolar lavage fluid, etc.), PCR products, cfDNA, cDNA, and salmon gDNA can all be successfully released from the library after the establishment of different types of samples, and the library output is>1 ug.

b: EDTA compatibility
EDTA with a final concentration of 0-3.5 mM in a tolerable system (the following figure shows the results after 300 ng nucleic acid interruption purification).
Reagent Composition&Description