Universal RNA-seq Library Prep Kit for Illumina

Product Description:
Compatible with Total RNA of various samples for corresponding RNA library construction. Universal RNA seq Library Prep Kit for Illumina is a specialized transcriptome library construction kit specifically developed for Illumina high-throughput sequencing platform. The kit contains two types of cDNA double stranded synthesis buffers, which can be selected as regular transcriptome or strand specific transcriptome for library construction as needed. The reagent kit combines two chain synthesis and end repair dA Tailing into one step, without the need for purification steps, greatly simplifying the operation process and shortening the library building time. The optimized reaction system improves the efficiency of library transformation, is compatible with lower initial input, and has uniform coverage for RNA with different initial amounts. This reagent kit utilizes magnetic bead sorting to quickly obtain a library of specific length, which can meet the personalized needs of different experiments. All enzymes and buffer solutions contained in the kit have undergone strict quality control and functional verification, greatly ensuring the stability and repeatability of library construction.

Product application:
RNA library construction; Gene expression analysis; Single nucleotide variation analysis; Variable shear analysis; Fusion gene detection; Target Transcriptome Analysis

Product advantages:
1. Fast: Simple operation process reduces the time for transcriptome library construction to 3 hours
2. Compatibility: For low initial volume and degraded samples, there is a higher success rate of database construction
3. High quality: Excellent sequencing data quality, resulting in more uniform coverage of transcriptional regions

Reagent Cases
1. Wide species compatibility
After extracting Total RNA from animals (human blood, 293T cells), bacteria (Escherichia coli), and plants (Arabidopsis, soybeans), N406 (animal rRNA removal product), N407 (bacterial rRNA removal product), N408 (blood rRNA removal product), and N409 (plant rRNA removal product) were selected for rRNA removal according to different species. Subsequently, following the library construction process of NR605, a chain specific transcriptome library was constructed. Use Qubit to detect library concentration.
2. For degradation samples, with a higher success rate of database construction
After extracting Total RNA from low-quality FFPE samples, N406 (animal rRNA removal product) was selected for rRNA removal. Subsequently, following the library construction process of NR605, a chain specific transcriptome library was constructed. The results showed that for FFPE samples of different qualities, NR605 was able to successfully build the database.
3. High data uniformity
The results showed that the libraries constructed using different transcriptome libraries showed high consistency in sequencing homogeneity.
4. Relatively rich gene detection numbers
The results showed that for the same sequencing quantity, using different transcriptome libraries to prepare product constructed libraries could obtain a relatively rich number of gene detections; But for low initial input, NR605 has a richer number of gene detections.

Composition and Description