Uracil DNA glycosylase (UNG enzyme)
UNG enzyme can selectively hydrolyze and break uracil glycosidic bonds in double or single stranded DNA containing dU, but has no activity on RNA. It is mainly used for anti contamination of PCR amplification products. This UNG enzyme is a recombinant protease expressed and purified from E. coli.
Definition of enzyme activity units:
The amount of enzyme required to release 1nmolde of dU from the substrate of double stranded or single stranded DNA containing dU under 37 ℃ reaction for 1 hour is one active unit.
Enzyme storage buffer:
20 mM Tris HCl buffer containing 1 mM EDTA, 1 mM DTT, 50 mM NaCl and 50% glycol (v/v), pH 7.5
Instructions for use:
The reaction buffer and enzyme storage solution for Taq enzyme and UNG enzyme can be used interchangeably in a 50uL reaction system, typically adding 0.2-1U.
Enzyme purity:
>95%, as determined by SDS-PAGE (no activity of DNA endonuclease and exonuclease detected), UNG enzyme completely lost its activity after 2 minutes of treatment at 94 ℃.
Saving conditions:
-20 ° storage
