Medium for Isolation and Cultivation of Enterobacteriaceae
This selective differential culture medium is specially formulated for the isolation and cultivation of Enterobacteriaceae. It suppresses most Gram-positive bacteria while allowing target enteric bacteria to grow, and differentiates glucose-fermenting strains by colony color.
- Product specification: 250 g/bottle
- Shelf life: 3 years
Formula Composition (per Liter)
| Ingredient |
Content |
| Yeast extract powder |
3.0 g |
| Peptone |
7.0 g |
| Sodium chloride |
5.0 g |
| Glucose |
10.0 g |
| No.3 bile salt |
1.5 g |
| Crystal violet |
0.002 g |
| Neutral red |
0.03 g |
| Agar |
12.0 g |
| Final pH |
7.4 ± 0.2 at 25 ℃ |
Inspection Principle
Peptone and yeast extract powder supply carbon, nitrogen sources and trace elements for bacterial growth. Glucose serves as fermentable carbohydrate. Sodium chloride maintains proper osmotic balance. No.3 bile salt and crystal violet inhibit Gram-positive bacteria, including Enterococcus faecalis. Neutral red is the pH indicator. Strains that ferment glucose produce acid, turning neutral red and forming red colonies.
Directions for Use
Weigh 39.5 g of the medium powder, add to 1000 mL distilled water and heat to boiling for dissolution. Boiling time shall not exceed 2 minutes. Cool to approximately 50 ℃ and pour into sterile plates. Autoclaving is not required.
